Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV

In order to develop new vaccine candidate of Equine infectious anema virus(EIAV)，env gene of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the downstream of pE／L promoter of pSC65 vector,separately．The resulted recombinant vaccinia viruses were screened out by homologous recombination in TK region and the positive clone was confirmed with blue plaque assay．Protein expression was examined by W estern blot．It was shown that the recombinant vaccinia viruses successfully expressed the complete EIAV Env proteins an d induced significan t cellular an d humoral immune responses in the Balb／c mi ce by intramuscular inoculation． CTL specific lysis is 28％ at most．Th e study will provide importan t elements for development of new EIAV genetic engineering vaccine．