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Volume 18 Issue 5
October 2003
    Citation: LI Yong—dan, ZHAO Chao—yang, WANG Li—ying. Preliminary Study on the Enhancing Factor of Oedaleus asiaticus Entomopoxvirus .VIROLOGICA SINICA, 2003, 18(5) : 478-481.
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    Preliminary Study on the Enhancing Factor of Oedaleus asiaticus Entomopoxvirus

    • 1.

      Entomology Department,China Agricultural University,Beijing 100094，China

    • Available online: 05 October 2003
    • Abstract：Oedaleus asiaticus entomopoxvirus(DaEPV) contains a factor which enhances the infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis．When the spheroids of OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged，the enhancing activity of the supernatant was 2．5 times higher than in the pellet．The enhancing activity of spheroid proteins depended on the alkali used to dissolve the spheroids．The greatest activity was detected with spheroid alkaline solution，followed with 0．02 mol／L NaOH，and none with 8 mol／L urea．Spheroid proteins were fractionated on a sephadex G一200 column．Two fractions were obtained an d the second fraction had the enhancing activity．SDS—PAGE indicated the molecular weight of the active fraction was40 kDa．
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      Preliminary Study on the Enhancing Factor of Oedaleus asiaticus Entomopoxvirus

      • 1. Entomology Department,China Agricultural University,Beijing 100094，China

      Abstract: Abstract：Oedaleus asiaticus entomopoxvirus(DaEPV) contains a factor which enhances the infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis．When the spheroids of OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged，the enhancing activity of the supernatant was 2．5 times higher than in the pellet．The enhancing activity of spheroid proteins depended on the alkali used to dissolve the spheroids．The greatest activity was detected with spheroid alkaline solution，followed with 0．02 mol／L NaOH，and none with 8 mol／L urea．Spheroid proteins were fractionated on a sephadex G一200 column．Two fractions were obtained an d the second fraction had the enhancing activity．SDS—PAGE indicated the molecular weight of the active fraction was40 kDa．

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