Expression of Non—structural Protein S6 of Rice dwarf virus in E coil and Preparation of Its Antiserum

Abstract： gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT．PCR and then cloned into pGEM—Teasy．Sequence analysis showed that its nucleotide sequence had high identity to a Japan ese isolate of RDV an d contmned high percent of rare codons． l6 gene was expressed m inclusion body with high yield in E．coli after being subcloned into expression vector pGEX一6P—1．Immune rabbit witll S6 protein obtained the antiserum．The an tiserum having a titer of l：3000 tested by ELISA Can specifically react wim the expressed S6 protein．Our study confn-med that the an tiserum could be used to detect S6 protein in RDV-infected rice tissues．