Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain

To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain，to express E2 protein in BHl(2 l cells，and to analyze the antigenic sites of E2 protein of RV JR23 strain．E2 gene of RV JR23 strain was amplified bv I PCR．The PCR product was cloned into the expression vector pBluescript II S and transformed into E．coli JM 109．an d the recombinant plasmid was transfected into BHK21 cells with recombinant vaccinia viru s VTF一7．The expression was detected by immunohistochemistry an d indirect immunofluorescence．The recombinan t plasmid contained glycoprotein E2 gene．and expressed well in BHK2 l cells．The expressed E2 protein mainly accumulated in plasma near the nuclei．Th ere are immunological epitopes of E2 protein on RV JR23 virions．The results showed that RV JR23 E2 transient expression system was successfully constructed． This study ofers the basis for investigation of the relationship between the structure an d fu nction of RV proteins，and interaction of viru s an d host cells．