Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus

Abstract：Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides．As pe sticides，both viruses have very narrow host range and Can not make cross infection．Trying tO generate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection，we found that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR．which could be blocked by SeM NPV，but apoptosis did not occur in Se一30 1，a cell line also from exigua．W hen infected Se-UCR with 5M OI，theapoptoticbodywasIn’stfound at 12hpi，obvious apoptosis couldbe observedat 24hpi， at the end almost all ceHs were dead at 72 hpi．Those observations were confu-med by genome DNA fragmen-ration analysis．Dot blot an alysis indicated that SeMNPV could also help HaSNPV genome DNA an d then virus repficafion in Se-UCR cells，but not in Se-301．