Citation: ZHAO Chao—yang, W ANG Li-ying, LI Yong-dan, YUN Gui-ling. Cloning and Analysis of Oedaleus asiaticus Entomopoxvirus Spheroidin Gene .VIROLOGICA SINICA, 2003, 18(6) : 593-596.

Cloning and Analysis of Oedaleus asiaticus Entomopoxvirus Spheroidin Gene

  • Available online: 25 December 2003
  • Abstract:TIle coding region of Oedaleus asiaticus EPV spheroidin gene was obtained by PCR.and this fragment Was subsequently cloned,seq uenced an d an alyzed.Tlle data shows OaEPV sph coding re on is 2967bp in size which encodes a ll l l(Da protein.Homology an alysis demonstrated that 0aEPV is closer to Orthoptcran EPVS than Lepidopteran an d Coleopteran EPVS.Phylogenetic tree derived from all known EPVS spheroidin gene indicates that the order of the EPV host is in relationship with the genus of corresponding EPV.which agres wit|l a recent point that “Lepidopteran EPVs an d Orthopteran EPVS should be divided into two distinct genus”.Hydrophobicity plots of these kn own speroidin show that the more distan t relationship of the orders the viruses hOsts be long to.the more diverse of the property of EPV spheroidin to each other.It may be explained as the result of CO.evolutionbe tweentheEPVSan dtheirhos

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    Cloning and Analysis of Oedaleus asiaticus Entomopoxvirus Spheroidin Gene

    • 1. Entomology Department,China Agricultural University,Beijing 100094,China

    Abstract: Abstract:TIle coding region of Oedaleus asiaticus EPV spheroidin gene was obtained by PCR.and this fragment Was subsequently cloned,seq uenced an d an alyzed.Tlle data shows OaEPV sph coding re on is 2967bp in size which encodes a ll l l(Da protein.Homology an alysis demonstrated that 0aEPV is closer to Orthoptcran EPVS than Lepidopteran an d Coleopteran EPVS.Phylogenetic tree derived from all known EPVS spheroidin gene indicates that the order of the EPV host is in relationship with the genus of corresponding EPV.which agres wit|l a recent point that “Lepidopteran EPVs an d Orthopteran EPVS should be divided into two distinct genus”.Hydrophobicity plots of these kn own speroidin show that the more distan t relationship of the orders the viruses hOsts be long to.the more diverse of the property of EPV spheroidin to each other.It may be explained as the result of CO.evolutionbe tweentheEPVSan dtheirhos

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