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Volume 18 Issue 6
December 2003
    Citation: CHEN M ing—ruing, WU Dong, YUAN Li, CHEN Xin—wen, HU Zhi—hong. Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies .VIROLOGICA SINICA, 2003, 18(6) : 597-602.
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    Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies

    • 1.

      Joint-lab ofInvertebrate Virology and Key Laboratory of Molecular Virology,Wuhan Institute of Virology,Chinese AcademyofSciences,Wuhan430071，China

    • Available online: 25 December 2003
    • Abstract：Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the genome DNA of HaSNPV．The PCR products were cloned into pBluescript KS(+)plasmid．The genes were introd uced into the expression vector pProExHTb．After IFFG induction．the E coli DH5a strain containing each of the recombinant plasmids expressed proteins with molecular weights of 32 kDa、64 kDa an d 58 kDa,respectively,which were in agreement with the prospeetation．Th e purified recombinant proteins were used to immune the rabbits separately．Western blot an alysis using the multiclonal an tibodies derived from the rabbits indicated that these an tibodies could react specifically with thetargetproteinsan dwere suitableto beusedforfurtherfunctional an alysisofthebrogenes．
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      Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies

      • 1. Joint-lab ofInvertebrate Virology and Key Laboratory of Molecular Virology,Wuhan Institute of Virology,Chinese AcademyofSciences,Wuhan430071，China

      Abstract: Abstract：Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the genome DNA of HaSNPV．The PCR products were cloned into pBluescript KS(+)plasmid．The genes were introd uced into the expression vector pProExHTb．After IFFG induction．the E coli DH5a strain containing each of the recombinant plasmids expressed proteins with molecular weights of 32 kDa、64 kDa an d 58 kDa,respectively,which were in agreement with the prospeetation．Th e purified recombinant proteins were used to immune the rabbits separately．Western blot an alysis using the multiclonal an tibodies derived from the rabbits indicated that these an tibodies could react specifically with thetargetproteinsan dwere suitableto beusedforfurtherfunctional an alysisofthebrogenes．

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