Harpin Expression and Induction of Bioactivity to Anti-TMV Infection
Abstract: Abstract:Harpin gene A,B,C and D fragments of Erwinia amylovora(Ea)were synthesized.The full length Harpin gene was constructed by ligation,and then cloned into pET-23(+).Th e fusion Harpin protein Was expressed in E.coli BL2 1,purified by His Bind Resin Ni”chromatography and subjected to SDS—PAGE an d Western blot.The Mr of the Harpin was 45 kDa.5pL 301ag/mE Harpin induced hypersensitive response on tobacco or capsicum leaves by using punctures or injection along nervation in laminae respectively.Tobacco an d capsicum were treated with 30pg/mE Harpin protein by spraying over the plant,and then Tobacco mosaic virus(TMV)was inoculated in the plants by mechanical inoc ulation after 5 days.Th e TM V concentration in leaves was determined by ELISA.TM V infection in tobacco an d capsicum treated with Harpin was repressed to a certain extent.