XU Li, WANG Ling, SU Xin, WAN G, W U, CHEN Pu.Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies .VIROLOGICA SINICA, 2003, 18(6): 614-615.
Citation:
XU Li, WANG Ling, SU Xin, WAN G, W U, CHEN Pu.
Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies .VIROLOGICA SINICA, 2003, 18(6)
: 614-615.
Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies
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1.
1．KeyLaboratoryofAnimalDiseaseDiagnosisandImmunologyNanjingAgricultural University,Nanjing 210095． China
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2.
Department ofAnimal Science．Agricultural College ofNingxia University．Yinchuan 750105．China 3 Animal Quarantine Institute of Agriculture Ministry,Qingdao 266032，China
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Available online:
25 December 2003
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Abstract
Abstract：According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome V／ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank， two pairs of primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR． As a result， a rapid molecular diagn osis with high specificity an d accuracy was set up．The result indicated that PRRSV Was detected from 11 out of 33 samples， PRV was detected from 9 out of 33 samples， and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples， the rate of co-infection Was at24．2％ ．
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References
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Proportional views
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