Immunological Characteristics of a 27kDa Protein in the Bovine Sera
Abstract: To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.