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Volume 19 Issue 1
February 2004
    Citation: SHAO Xian-an, SHEN Yan, XU Wei, WANG Ying, HU Xin-mei, XIONG Si-dong*. Immunological Characteristics of a 27kDa Protein in the Bovine Sera .VIROLOGICA SINICA, 2004, 19(1) : 6-9.
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    Immunological Characteristics of a 27kDa Protein in the Bovine Sera

    • 1.

      Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College, Fudan University

    • Available online: 20 February 2004
    • To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.
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      Immunological Characteristics of a 27kDa Protein in the Bovine Sera

      • 1. Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College, Fudan University

      Abstract: To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.

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