Citation: LI Lan-juan, LU Yi-yu, WENG Jing-qing, YAN Ju-ying, WU Nan-ping, WO Jian-er, SHI Wen, GONG Li-ming, ZHU Han-ping, GE Qiong, LI Min-hong, LU Qun-ying, CHENG Su-yun, ZHANG Yan-jun, YAO Ping-ping, MEI Ling-ling, WANG Zhi-gang, FEN Yan, MAO Hai-yan. Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province .VIROLOGICA SINICA, 2004, 19(1) : 14-17.

Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

  • Corresponding author: LU Yi-yu, 
  • Available online: 20 February 2004
  • Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.

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    Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

      Corresponding author: LU Yi-yu,
    • 1. 1. Medical College of Zhejiang University, Hangzhou 310009, China
    • 2. Zhejiang Center for Disease Prevention and Control

    Abstract: Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.

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