Serving the Society Since 1986
Advanced Search
Volume 19 Issue 1
February 2004
    Citation: LI Lan-juan, LU Yi-yu, WENG Jing-qing, YAN Ju-ying, WU Nan-ping, WO Jian-er, SHI Wen, GONG Li-ming, ZHU Han-ping, GE Qiong, LI Min-hong, LU Qun-ying, CHENG Su-yun, ZHANG Yan-jun, YAO Ping-ping, MEI Ling-ling, WANG Zhi-gang, FEN Yan, MAO Hai-yan. Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province .VIROLOGICA SINICA, 2004, 19(1) : 14-17.
    shu

    Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

    • 1.

      1. Medical College of Zhejiang University, Hangzhou 310009, China

    • 2.

      Zhejiang Center for Disease Prevention and Control

    • Corresponding author: LU Yi-yu, 
    • Available online: 20 February 2004
    • Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4294) PDF downloads(1220) Cited by()

    Related
    Proportional views

      Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

        Corresponding author: LU Yi-yu,
      • 1. 1. Medical College of Zhejiang University, Hangzhou 310009, China
      • 2. Zhejiang Center for Disease Prevention and Control

      Abstract: Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return