Citation: XU Chuan-tian, FAN Wei-xing*, ZHAO Hong-kun. Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene .VIROLOGICA SINICA, 2004, 19(1) : 27-31.

Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene

  • Available online: 20 February 2004
  • 10 influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned and sequenced and compared with other H9N2 isolates of avian and swine in GenBank. The results indicated that the isolate Sw/SD/1/2003 (H9N2) was similar to Ck/GX/1999 (H9N2) and Ck/YN/2000 (H9N2 ) in HA gene; Sw/SD/1/2003 might come from an avian influenza H9N2 subtype according to the phylogenetic analysis of HA genes; the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, but the sequences of all other H9N2 subtypes of porcine isolates and avian isolates are R-S-S-R-G.

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    Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene

    • 1. College of Animal Science, Shandong Agricultural University, Taian 271018, China

    Abstract: 10 influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned and sequenced and compared with other H9N2 isolates of avian and swine in GenBank. The results indicated that the isolate Sw/SD/1/2003 (H9N2) was similar to Ck/GX/1999 (H9N2) and Ck/YN/2000 (H9N2 ) in HA gene; Sw/SD/1/2003 might come from an avian influenza H9N2 subtype according to the phylogenetic analysis of HA genes; the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, but the sequences of all other H9N2 subtypes of porcine isolates and avian isolates are R-S-S-R-G.

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