Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus

By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus（SeNPV）has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin interacted with IAP2 or IAP3 weakly, and ubiquitin was transferred to IAP2 or IAP3 through ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) in yeast. IAPs encoded by SeNPV may be the ubiquitin ligase (E3).