Serving the Society Since 1986
Advanced Search
Volume 19 Issue 1
February 2004
    Citation: WANG Fu-xiang, SUN Yong-tao*, WANG Lin-xu, WANG Jiu-ping, WANG Ping-zhong, BAI Xue-fan, HUANG Chang-xing. Construction and Expression of HIV-1 Env Eukaryotic Expression Vector .VIROLOGICA SINICA, 2004, 19(1) : 70-72.
    shu

    Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

    • 1.

      Center of Diagnosis and Treatment for Infectious Diseases of PLA. Tangdu Hospital, The Fourth Military Medical Univercity, Xi′an 710038, China

    • Available online: 20 February 2004
    • To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(3948) PDF downloads(1192) Cited by()

    Related
    Proportional views

      Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

      • 1. Center of Diagnosis and Treatment for Infectious Diseases of PLA. Tangdu Hospital, The Fourth Military Medical Univercity, Xi′an 710038, China

      Abstract: To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return