Citation: WANG Fu-xiang, SUN Yong-tao*, WANG Lin-xu, WANG Jiu-ping, WANG Ping-zhong, BAI Xue-fan, HUANG Chang-xing. Construction and Expression of HIV-1 Env Eukaryotic Expression Vector .VIROLOGICA SINICA, 2004, 19(1) : 70-72.

Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

  • Available online: 20 February 2004
  • To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.

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    Construction and Expression of HIV-1 Env Eukaryotic Expression Vector

    • 1. Center of Diagnosis and Treatment for Infectious Diseases of PLA. Tangdu Hospital, The Fourth Military Medical Univercity, Xi′an 710038, China

    Abstract: To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.

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