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Volume 19 Issue 1
February 2004
    Citation: DING Ming, FANG Qi, ZHANG Li-zhen, YANG Lu-ming, Wang Ji-ling, LI Yan-fang, ZHANG Zhong-kai. Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province .VIROLOGICA SINICA, 2004, 19(1) : 76-78.
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    Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province

    • 1.

      1. Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223,China

    • 2.

      Honghe Cigarette Factory, Mile 652300, China

    • Corresponding author: ZHANG Zhong-kai, 
    • Available online: 20 February 2004
    • Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B, TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain.
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      Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province

        Corresponding author: ZHANG Zhong-kai,
      • 1. 1. Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223,China
      • 2. Honghe Cigarette Factory, Mile 652300, China

      Abstract: Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV coat protein gene was amplified using IC-PCR. The products of PCR were cloned and sequenced. The nucleotide and amino acid sequences of the coat protein of the five isolates were found to be very similar each other and have more than 90% sequence identity with TMV-U1, TMV-B, TMV-P, TMV-FUJIAN, although minor differences existed among them. The results showed that the five isolates from Yunnan province belong toTMV-U1 strain.

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