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Volume 19 Issue 2
April 2004
    Citation: YUAN Yu-hua, BI Chang-hao, LI Ju, WANG Xue-qian, GENG Yun-qi, CHENG Qi-min. Low-temperature Induced Expression of HIV gp41 Gene Segments .VIROLOGICA SINICA, 2004, 19(2) : 179-181.
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    Low-temperature Induced Expression of HIV gp41 Gene Segments

    • 1.

      College of Life Science, Nankai University, Tianjin 300071,China

    • Corresponding author: CHENG Qi-min, 
    • Available online: 20 April 2004
    • Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.
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      Low-temperature Induced Expression of HIV gp41 Gene Segments

        Corresponding author: CHENG Qi-min,
      • 1. College of Life Science, Nankai University, Tianjin 300071,China

      Abstract: Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.

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