Citation: LUO Bao-jun, ZHANG Qiang, YANG Qian, DING Mei, LIU Shu-ling, HANG Chang-shou, WANG Hai-tao, ZHOU Yu-sen*. Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector .VIROLOGICA SINICA, 2004, 19(3) : 224-227.

Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector

  • Available online: 20 June 2004
  • In order to study the immune responses of Hantaan virus envelope glycoproteins G1 and G2 in adenovirus expression system, G1 and G2 gene were amplified from Hantaan virus 76-118 and cloned to the plasmid pAdTrackCMV. Positive clones pAdTrackCMV-G1 and G2 were determined with restriction enzyme digestion. The recombinant adenovirus rAdeasy-G1 and rAdeasy-G2 were generated by bacterial homologous recombination with adenoviral plasmid pAdeasy-1 in E.coli BJ5183. After amplification in the 293 cell, the recombinant viruses were immuned to the Balb/c mice. The antibody reaction were detected by ELISA and Immunofluorescence Assay. Six rAdeasy-G1 immuned mice and four rAdeasy-G2 immuned mice produce antibody which can specifically react with the Hantaan virus. These results suggested a possible application of adenovir- us expression system.

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    Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector

    • 1. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071 ,China

    Abstract: In order to study the immune responses of Hantaan virus envelope glycoproteins G1 and G2 in adenovirus expression system, G1 and G2 gene were amplified from Hantaan virus 76-118 and cloned to the plasmid pAdTrackCMV. Positive clones pAdTrackCMV-G1 and G2 were determined with restriction enzyme digestion. The recombinant adenovirus rAdeasy-G1 and rAdeasy-G2 were generated by bacterial homologous recombination with adenoviral plasmid pAdeasy-1 in E.coli BJ5183. After amplification in the 293 cell, the recombinant viruses were immuned to the Balb/c mice. The antibody reaction were detected by ELISA and Immunofluorescence Assay. Six rAdeasy-G1 immuned mice and four rAdeasy-G2 immuned mice produce antibody which can specifically react with the Hantaan virus. These results suggested a possible application of adenovir- us expression system.

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