Citation: ZHANG Jie, YE Lin-bai, ZENG Ying-chun, YANG Fan, LIU Jing. For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells .VIROLOGICA SINICA, 2004, 19(3) : 242-244.

For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells

  • Corresponding author: YE Lin-bai, 
  • Available online: 20 June 2004
  • To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing 1%~3% horse serum. Virus were harvested at the fifth and eighth days and used for preparing antigens by concentration, inactivation and centrifugation. The titers of rabies virus in the culture were reached above 7.0 logLD50/mL .The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.

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    For Preparing the Antigen of Rabies Immunoglobulin in Vero Cells

      Corresponding author: YE Lin-bai,
    • 1. 1. Institute of virology,Wuhan University,Wuhan 430072,China
    • 2. Wuhan lnstitute of biological products, Wuhan 430060, China

    Abstract: To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing 1%~3% horse serum. Virus were harvested at the fifth and eighth days and used for preparing antigens by concentration, inactivation and centrifugation. The titers of rabies virus in the culture were reached above 7.0 logLD50/mL .The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.

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