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Volume 19 Issue 3
June 2004
    Citation: ZHAO Wen-ming, DING Jia-bo, JIANG Shi-jin, CUI Zhi-Zhong. The Promoting Function of Long Terminal Repeat from Reticuendotheliosis Virus .VIROLOGICA SINICA, 2004, 19(3) : 255-258.
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    The Promoting Function of Long Terminal Repeat from Reticuendotheliosis Virus

    • 1.

      1. College of Animal Husbandy and Veterinary Medicine, Yangzhou University, Yangzhou 22500, China

    • 2.

      College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China

    • Corresponding author: ZHAO Wen-ming, 
    • Available online: 20 June 2004
    • The long terminal repeat (LTR) of Reticuendotheliosis virus (REV) was amplified by PCR technique and cloned into pUC18 vector at the sites of EcoRI and SacI, and the BGH polyadenylation signal sequence was cloned at the sites of Sph I and Hind Ⅲ as a terminor. The positive clone was named pUC-LTR, which was used as a basic expressing vector to validate the activity of LTR. Green fluorescent protein (GFP) and REV envelope glycoprotein 90 (gp90) gene was cloned at the downstream of LTR in the pUC-LTR vector respectively as a reporter. These two recombinants pUC-LTR-GFP and pUC-LTR-gp90 were then transfected into Chicken Embryo Fibroblast (CEF) cells. 48h after the transfection, we could detect the expression of GFP and gp90. This study shows the LTR sequence could be used as a promoter to construct expressing vectors.
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      The Promoting Function of Long Terminal Repeat from Reticuendotheliosis Virus

        Corresponding author: ZHAO Wen-ming,
      • 1. 1. College of Animal Husbandy and Veterinary Medicine, Yangzhou University, Yangzhou 22500, China
      • 2. College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China

      Abstract: The long terminal repeat (LTR) of Reticuendotheliosis virus (REV) was amplified by PCR technique and cloned into pUC18 vector at the sites of EcoRI and SacI, and the BGH polyadenylation signal sequence was cloned at the sites of Sph I and Hind Ⅲ as a terminor. The positive clone was named pUC-LTR, which was used as a basic expressing vector to validate the activity of LTR. Green fluorescent protein (GFP) and REV envelope glycoprotein 90 (gp90) gene was cloned at the downstream of LTR in the pUC-LTR vector respectively as a reporter. These two recombinants pUC-LTR-GFP and pUC-LTR-gp90 were then transfected into Chicken Embryo Fibroblast (CEF) cells. 48h after the transfection, we could detect the expression of GFP and gp90. This study shows the LTR sequence could be used as a promoter to construct expressing vectors.

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