Selection of Differentially Expressed Signal Transduction-Associated Genes of Integrin in Hantavirus Infected Cells

To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 virus. Total RNAs were extracted from infected and normal control group 6h, 24h and 96h post infection, respectively. During reverse transcription, the cDNAs were labeled by Cy5-dUTP and Cy3-dUTP. The labeled cDNAs hybridized with the Biostar-ⅡH gene chip. After hybridization, the fluorescence signal was analyzed by scanning. It showed that the expression level of most (113/150) Hantavirus receptor and signal transduction-associated genes was normal, however, 37 genes presented obvious change. Among them, expressions of 31 genes changed at 6h post infection. 13 genes were down-regulated, and 18 up-regulated. However, 5 and 4 genes expressed diversely at 24h and 96h post infection respectively. Furthermore, some integrin signal transduction pathway-associated genes such as the gene of some related protein kinases were up-regulated at 6h post infection. The gene of enhancer of filamentation 1 was down-regulated at 6h and 96h post infection. The gene of microtubule-associated protein 1A was up-regulated at 6h post infection, but down-regulated at 24h and 96h post infection. These indicate that Hantavirus infection is related to integrins.