Serving the Society Since 1986
Advanced Search
Volume 19 Issue 4
August 2004
    Citation: DENG Yu-xiu, JIANG Ping, LI Yu-feng, WANG Xian-wei, JIANG Wen-ming, TANG Jing-yuan. Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP .VIROLOGICA SINICA, 2004, 19(4) : 398-400.
    shu

    Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

    • 1.

      Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

    • Corresponding author: JIANG Ping, 
    • Available online: 25 August 2004
    • Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(3763) PDF downloads(992) Cited by()

    Related
    Proportional views

      Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Eastern China with RT-PCR-RFLP

        Corresponding author: JIANG Ping,
      • 1. Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095,China

      Abstract: Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length polymorphism (RFLP) patterns for enzymes MluⅠ, HincⅡand SacⅡwere determined on these ORF5 genes. The RFLP pattern obtained from 39 isolates was 2-2-1(78%), 3 isolates was 1-1-1(6%) and 8 isolates identified in 2003 was 1-3-1 (16%). The results showed PRRSV strains have variations and existed no less than 3 gene subtypes in the area.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return