Serving the Society Since 1986
Advanced Search
Volume 19 Issue 5
October 2004
    Citation: SONG Yan, LI Yi-jing*, WEI Li-li, YU Xiao-long, FAN Chen, SHI Dong-fang. Expression of vp4 Gene from Porcine Rotavirus in E. coli .VIROLOGICA SINICA, 2004, 19(5) : 462-466.
    shu

    Expression of vp4 Gene from Porcine Rotavirus in E. coli

    • 1.

      Molecular Virology Veterinary Medicine College, Northeast Agricultural University, Harbin 150030, China

    • Available online: 20 October 2004
    • A pair of primers was used in cloning and sequence analysis of vp4 gene from Porcine rotavirus JL94 isolated in China. According to the idea that 5’ segments of VP4 (1bp～750bp) principally control the activity of vp4 gene, a pair of another primers was used in cloning and obtained major antigen sites. The gene was cloned into the expression plasmid pGEX-6P-1. The recombinant plasmid VP4-pGEX-6P-1 was transformed into E. coli BL21(DE3)plays and induced with IPTG. Homology of amino acids between CRW-8 strain and BEN-307 strain is 96.98% and 98.05% respectively. The product of the VP4 gene was 26% of total bacterial protein of BL21. Western blot test and neutralization test circumstantiate the protein of expression has biological activity.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4208) PDF downloads(909) Cited by()

    Related
    Proportional views

      Expression of vp4 Gene from Porcine Rotavirus in E. coli

      • 1. Molecular Virology Veterinary Medicine College, Northeast Agricultural University, Harbin 150030, China

      Abstract: A pair of primers was used in cloning and sequence analysis of vp4 gene from Porcine rotavirus JL94 isolated in China. According to the idea that 5’ segments of VP4 (1bp～750bp) principally control the activity of vp4 gene, a pair of another primers was used in cloning and obtained major antigen sites. The gene was cloned into the expression plasmid pGEX-6P-1. The recombinant plasmid VP4-pGEX-6P-1 was transformed into E. coli BL21(DE3)plays and induced with IPTG. Homology of amino acids between CRW-8 strain and BEN-307 strain is 96.98% and 98.05% respectively. The product of the VP4 gene was 26% of total bacterial protein of BL21. Western blot test and neutralization test circumstantiate the protein of expression has biological activity.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return