Citation: JIA Yun, ZHANG Su-fang, ZHOU Bin, ZHENG Qi-sheng, ZHAO Yu-jun, CHEN Pu-yan. Quick Detection for Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus Mixed Infection and Regional Epidemiology Analysis .VIROLOGICA SINICA, 2004, 19(5) : 514-516.

Quick Detection for Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus Mixed Infection and Regional Epidemiology Analysis

  • Corresponding author: CHEN Pu-yan, 
  • Available online: 20 October 2004
  • Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C strain from GenBank, RT-PCR method was established to detect these two viruses at the same time. With this method, 87 tissue samples of 24 pig farms from Jiangsu, Shanghai and Fujian were tested. Among these samples 75 were PRRS positive, 56 were CSF positive, 53 were both PRRS and CSF positive, which amounts to 60.9 percent of all samples. Combined with regional epidemiology analysis, We concluded that samples from Fujian mainly mixed infected with PRRSV and CSFV, but samples from Jiansu simply infected with PRRSV.

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    Quick Detection for Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus Mixed Infection and Regional Epidemiology Analysis

      Corresponding author: CHEN Pu-yan,
    • 1. 1.Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing 210095, China
    • 2. Department of Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161,China

    Abstract: Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C strain from GenBank, RT-PCR method was established to detect these two viruses at the same time. With this method, 87 tissue samples of 24 pig farms from Jiangsu, Shanghai and Fujian were tested. Among these samples 75 were PRRS positive, 56 were CSF positive, 53 were both PRRS and CSF positive, which amounts to 60.9 percent of all samples. Combined with regional epidemiology analysis, We concluded that samples from Fujian mainly mixed infected with PRRSV and CSFV, but samples from Jiansu simply infected with PRRSV.

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