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Volume 19 Issue 5
October 2004
    Citation: SONG Ai-jing, LI Xiou-hua, ZHANG Chun-tao, WANG You-chun*. Analysis of HIV Genotypes Isolated from Blood Donors in Beijing .VIROLOGICA SINICA, 2004, 19(5) : 517-519.
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    Analysis of HIV Genotypes Isolated from Blood Donors in Beijing

    • 1.

      Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

    • Available online: 20 October 2004
    • To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. The purified PCR products were cloned. The sequences were determined and analyzed. The results showed that 10 of 20 isolates were CRF BC, 9 of them were genotype B, 1 of them were CRF AE. 6 of 9 genotype B belong to B’(Thailand B), the remaining 3 isolates may belong to western genotype B. The results indicated that several different genotypes of HIV which are predominant in China may exist in Beijing. This result may be helpful for the development of HIV vaccine in Beijing.
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      Analysis of HIV Genotypes Isolated from Blood Donors in Beijing

      • 1. Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

      Abstract: To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. The purified PCR products were cloned. The sequences were determined and analyzed. The results showed that 10 of 20 isolates were CRF BC, 9 of them were genotype B, 1 of them were CRF AE. 6 of 9 genotype B belong to B’(Thailand B), the remaining 3 isolates may belong to western genotype B. The results indicated that several different genotypes of HIV which are predominant in China may exist in Beijing. This result may be helpful for the development of HIV vaccine in Beijing.

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