Citation: Li Yuan-chao, LU Feng-lin*, CAO Wei, ZUO Guo-wei, AI Jun-hua, JIN dan, HU Cheng-xiang. Study on B Cell Epitope and It’s Antigenicity of the L1 Capsid Protein of Human Papilloma Virus Type 6 .VIROLOGICA SINICA, 2004, 19(6) : 541-544.

Study on B Cell Epitope and It’s Antigenicity of the L1 Capsid Protein of Human Papilloma Virus Type 6

  • Available online: 20 December 2004
  • We analyzed and verificated the B-cell epitope of the L1 capsid protein of the Human papilloma virus type 6, and made it the base of producing new subunit peptide vaccine. Goldkey and PC/ Gene computer program were used to colligate and analyze the B-cell epitope of HPV6 L1. These peptides were synthesised and purified by Fmoc and HPLC methods, respectively. After that the peptides were emulsified with 0.2ml adjuvant, the blood serum antibody titer of mouse immuned by 50 μg peptides were detected. We found that, the B cell epitopes of the L1 protein of HPV-6 could be located at NO.425-439 and 486-500 positions. Those synthesized peptides have the ability to raise blood serum antibody titer enormously in vitro, and the antibody induced by these synthesized peptides could react with the supernatant of human papilloma tissue. We conclude that NO. 425-439 and 486-500 positions are predominat B-cell epitope of HPV 6 L1 protein. More work is under way to investigate whether these antibodies could perform functions.

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    Study on B Cell Epitope and It’s Antigenicity of the L1 Capsid Protein of Human Papilloma Virus Type 6

    • 1. Research Institute of Surgery & Daping Hospital, The Third Military Medical University, Chongqing

    Abstract: We analyzed and verificated the B-cell epitope of the L1 capsid protein of the Human papilloma virus type 6, and made it the base of producing new subunit peptide vaccine. Goldkey and PC/ Gene computer program were used to colligate and analyze the B-cell epitope of HPV6 L1. These peptides were synthesised and purified by Fmoc and HPLC methods, respectively. After that the peptides were emulsified with 0.2ml adjuvant, the blood serum antibody titer of mouse immuned by 50 μg peptides were detected. We found that, the B cell epitopes of the L1 protein of HPV-6 could be located at NO.425-439 and 486-500 positions. Those synthesized peptides have the ability to raise blood serum antibody titer enormously in vitro, and the antibody induced by these synthesized peptides could react with the supernatant of human papilloma tissue. We conclude that NO. 425-439 and 486-500 positions are predominat B-cell epitope of HPV 6 L1 protein. More work is under way to investigate whether these antibodies could perform functions.

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