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Volume 19 Issue 6
December 2004
    Citation: LI Zi-jian, JIN Ning-yi, ZHANG Li-su, JIANG Wen-zheng, ZHANG Hong-yong. Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c .VIROLOGICA SINICA, 2004, 19(6) : 559-562.
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    Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c

    • 1.

      1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China

    • Corresponding author: JIN Ning-yi, 
    • Available online: 20 December 2004
    • The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4＋and CD8＋ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P0.01), the numbers of spleen CD4+ and CD8+ T cells (P0.05) and the specific killing activities of spleen CTL (P0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.
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      Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c

        Corresponding author: JIN Ning-yi,
      • 1. 1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China

      Abstract: The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4＋and CD8＋ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P0.01), the numbers of spleen CD4+ and CD8+ T cells (P0.05) and the specific killing activities of spleen CTL (P0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.

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