Citation: DUAN Bing, ZHAO Shu-ling, ZHANG Hai-yuan, ZHANG Xiao-xia, XIAO Yu-zhou, TANG Xian-chun, PENG Hui-yin. cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus .VIROLOGICA SINICA, 2004, 19(6) : 627-631.

cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus

  • Corresponding author: PENG Hui-yin, 
  • Available online: 20 December 2004
  • The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.

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    cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus

      Corresponding author: PENG Hui-yin,
    • 1. 1. Key Lab of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences,Wuhan 430071,China
    • 2. The Graduate School of Chinese Academy of Sciences, Bejing 100039, China

    Abstract: The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.

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