cDNA Clone of Segment 8 and Prokaryotic Expression of Dendrolimus punctatus Wenshanensis cypovirus
Abstract: The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted into pET-28a expression vector and expressed in E.coli BL21. SDS-PAGE results showed that p44 gene has expressed.