Citation: REN Xue-feng, HU Zhi-hua, TAN Guo-lei, ZHOU Bing, XU Xue-qin, ZHENG Qi-sheng, Zhang Xiao-yong, CHEN De-sheng, CHEN Pu-yan*. Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg* .VIROLOGICA SINICA, 2004, 19(6) : 636-638.

Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg*

  • Available online: 20 December 2004
  • Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.

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    Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg*

    • 1. Key Laboratory of Animal Disease diagnosis and Immunology, Ministry of Agriculture at Nanjing Agricultural University, Nanjing, Jiangshu 210095, China

    Abstract: Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.

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