Citation: WEI Zhan-yong, CUI Bao-an, HUANG Ke-he, WANG Xue-bin, JIN Xi-xin, ZHANG Long-xian. Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro .VIROLOGICA SINICA, 2005, 20(1) : 33-36.

Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro

  • Corresponding author: HUANG Ke-he, 
  • Available online: 20 January 2005
  • Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. The results showed that production of NO was effectively induced in cell cultures treated with S-nitroso-N-acetylpenicllamine (SNAP) or L-arginine and replication of PPV was significantly inhibited by the addition of them in a dose-dependent manner.SNAP produced significantly higher amounts of NO and showed higher level of inhibitory effect on PPV replicaton than L-Arg at 100 and 200 μmol/L. Higher level of inhibition of viral production was also obtained when pretreated with SNAP at 6 h and 3 h before infection than added after infection,suggesting that the anti-PPV effects of NO occurred at the initial stages of PPV infection. In addition, the anti-PPV effects of L-arginine could be blocked by N~G-nitro-L-Arginine.

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    Inhibitory Effects of Nitric oxide on the Porcine Parvovirus infection in vitro

      Corresponding author: HUANG Ke-he,
    • 1. 

    Abstract: Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. The results showed that production of NO was effectively induced in cell cultures treated with S-nitroso-N-acetylpenicllamine (SNAP) or L-arginine and replication of PPV was significantly inhibited by the addition of them in a dose-dependent manner.SNAP produced significantly higher amounts of NO and showed higher level of inhibitory effect on PPV replicaton than L-Arg at 100 and 200 μmol/L. Higher level of inhibition of viral production was also obtained when pretreated with SNAP at 6 h and 3 h before infection than added after infection,suggesting that the anti-PPV effects of NO occurred at the initial stages of PPV infection. In addition, the anti-PPV effects of L-arginine could be blocked by N~G-nitro-L-Arginine.

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