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Volume 20 Issue 1
February 2005
    Citation: ZHU Yi-xin, ZHANG Xiao-guang, YANG Yi-shu, LIU Chang, QIAO Wen-tao, CHEN Qi-min, GENG Yun-qi. Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus .VIROLOGICA SINICA, 2005, 20(1) : 37-40.
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    Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus

    • 1.

    • Corresponding author: GENG Yun-qi, 
    • Available online: 20 January 2005
    • The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a soluble form. After purification, the matrix without fusion peptide was obtained in milligram magnitude. The capsid was also expressed and purified in ten-milligram magnitude. We used the dissolvable BIV CA to immunized rabbit and got antiserum 53 d after first injection. Western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein. The protein could be used as the antigen to produce the antiserum and used in protein-protein interaction analysis.
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      Expression of Matrix and Capsid Protein of Bovine Immunodeficiency Virus

        Corresponding author: GENG Yun-qi,
      • 1. 

      Abstract: The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a soluble form. After purification, the matrix without fusion peptide was obtained in milligram magnitude. The capsid was also expressed and purified in ten-milligram magnitude. We used the dissolvable BIV CA to immunized rabbit and got antiserum 53 d after first injection. Western blotting analysis showed that the antiserum could specifically recognize the native HIV CA protein. The protein could be used as the antigen to produce the antiserum and used in protein-protein interaction analysis.

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