Citation: LI Jian-na, XIANG Kai-jun, ZHOU Rong, DING Yong-qiang, BAI Pei-yong, ZHANG Yu-ping, ZHU Bing, ZENG Qi-yi. Expression of SARS Coronavirus Membrane Protein and its DNA Vaccine Construction .VIROLOGICA SINICA, 2005, 20(2) : 113-116.

Expression of SARS Coronavirus Membrane Protein and its DNA Vaccine Construction

  • Available online: 20 April 2005
  • To study the immunological speciality of M protein of SARS-CoV and analyze the feasibility and necessity of M protein as the component of the SARS vaccine. M protein of SARS-CoV was expressed in E.coli by pET-15b and pET-22b and purified by affinity resin as antigen. M gene was cloned into the eukaryotic secretory expression plasmid pSecTagB and named recombinant plasmid pSecM as DNA vaccine which will be used to immunize BALB/c mice to obtain the antiserum of M protein. The immune effect of the M-DNA vaccine was studied by the ELISA technique established with purified M protein. Both of the recombinant M proteins can be expressed in soluble form. When compared with the SARS-CoV ELISA kit produced by Huada Co.Ltd, the prokaryotic reconbinant M proteins could react specifically with the sera from immunized M-DNA mice and SARS correct diagnosis patients. The recombinant M protein could be used as an antigen to detect SARS, and the SARS-CoV M gene vaccine could induce specifically antibody. M protein could be

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    Expression of SARS Coronavirus Membrane Protein and its DNA Vaccine Construction

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    Abstract: To study the immunological speciality of M protein of SARS-CoV and analyze the feasibility and necessity of M protein as the component of the SARS vaccine. M protein of SARS-CoV was expressed in E.coli by pET-15b and pET-22b and purified by affinity resin as antigen. M gene was cloned into the eukaryotic secretory expression plasmid pSecTagB and named recombinant plasmid pSecM as DNA vaccine which will be used to immunize BALB/c mice to obtain the antiserum of M protein. The immune effect of the M-DNA vaccine was studied by the ELISA technique established with purified M protein. Both of the recombinant M proteins can be expressed in soluble form. When compared with the SARS-CoV ELISA kit produced by Huada Co.Ltd, the prokaryotic reconbinant M proteins could react specifically with the sera from immunized M-DNA mice and SARS correct diagnosis patients. The recombinant M protein could be used as an antigen to detect SARS, and the SARS-CoV M gene vaccine could induce specifically antibody. M protein could be

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