Inhibitory Effect of Arbidol Hydrochloride on Coxsackievirus B3 Group in vitro

To study the antiviral activity of Arbidol hydrochloride on Coxsackievirus Group B_3 (CVB_3), the antiviral effect of Arbidol hydrochloride were evaluated with virus inhibitory rates by MTT assay and 50% tissue culture infection dose (TCID_(50)) of the supernatant. The results showed that the viral inhibitory rates were up to 75.48%,45.68%,28.90% and 48.27% respectively in interrupting viral biosynthesis group, directly killing virus group, interfering in viral absorption and penetration groups(2h and 8h). In interrupting viral biosynthesis group,Arbidol hydrochloride could significantly inhibit cytopathic effect (CPE) of CVB_3-infected HEp-2 cells, increase survival rates of CVB_3-infected cells in dose dependent manner and decrease viral titers of the supernatant. It indicates that Arbidol hydrochloride has antiviral effect against Coxsackievirus group B3 (CVB_3).