AcMNPV P35 Inhibit Apoptosis Induced by HaSNPV in Tn-Hi5 Cell

Autographa californica multicapsid nucleopolyhedrovirus(AcMNPV) blocked the Tn-Hi5 cell apoptosis stimulated by Helicoverpa armigera nucleopolyhedrovirus (HaSNPV). Futhermore AcMNPV could rescued HaSNPV replication in Tn-Hi5 cell and produced infective virus. This means that HaSNPV extend the host rang with AcMNPV help. The transient expression assay indicated that Ac-p35 could significantly inhibit the Tn-Hi5 apoptosis induced by HaSNPV.A recombinant HaSNPV which overexpression of p35: vHap35, was constructed to futher analyse P35 function. vHap35 was used to infect Tn-Hi5 as we expected the Tn-hi5 cell did not undergo apoptosis. Anyhow, no infective virus BV was detected. Under the electronic microscope viral particles were observed, which probably are the ODVs. The results demonstrat that p35 is funtional as both apoptosis inhibitor and host range factor.