Citation: TANG Yomg, CHEN Huan-chun, HUANG Qin-feng, YAO Qing-xia, ZHAO Wu. The Construction of cDNA Library of Porcine Reproductive and Respiratory Syndrome Virus and the Sequence of Its Genome .VIROLOGICA SINICA, 2005, 20(2) : 203-205.

The Construction of cDNA Library of Porcine Reproductive and Respiratory Syndrome Virus and the Sequence of Its Genome

  • Available online: 20 April 2005
  • In this study, the RNA of PRRSV HN1 strain was isolated and the cDNA fragments were amplified by RT-PCR. These fragments were then inserted to T-vector and constructed the cDNA library. The genome of PRRSV HN1 strain was sequenced and submitted to the GenBank obtained the ID: AY457635. The homology between this HN1 and other strains was analyzed. HN1 strain has the lowest homology with the Netherlands isolate LV which is 56.0%, and has the highest homology with the USA isolate MLV and VR2332 which is 99.1% and 99.0% respectively. The HN1 strain and MLV and VR2332 are on the same evolution branch, it proved that the HN1 strain is North America strain. In China, the HN1 strain has the highest homology with Beijing isolate BJ-4 (99.0%) and the lowest homology with another Beijing isolate HB2 (89.0%).

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    The Construction of cDNA Library of Porcine Reproductive and Respiratory Syndrome Virus and the Sequence of Its Genome

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    Abstract: In this study, the RNA of PRRSV HN1 strain was isolated and the cDNA fragments were amplified by RT-PCR. These fragments were then inserted to T-vector and constructed the cDNA library. The genome of PRRSV HN1 strain was sequenced and submitted to the GenBank obtained the ID: AY457635. The homology between this HN1 and other strains was analyzed. HN1 strain has the lowest homology with the Netherlands isolate LV which is 56.0%, and has the highest homology with the USA isolate MLV and VR2332 which is 99.1% and 99.0% respectively. The HN1 strain and MLV and VR2332 are on the same evolution branch, it proved that the HN1 strain is North America strain. In China, the HN1 strain has the highest homology with Beijing isolate BJ-4 (99.0%) and the lowest homology with another Beijing isolate HB2 (89.0%).

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