Citation: ZENG Yan, LIU Long-ding, MA Shao-hui, Cun Wei, GUO Hong-xiong, LI Qi-han*. Analysis of siRNA Interfering in theα22 Immediate-early Gene of HSV-1 .VIROLOGICA SINICA, 2005, 20(3) : 221-224.

Analysis of siRNA Interfering in theα22 Immediate-early Gene of HSV-1

  • Available online: 20 June 2005
  • In this study, α22, an immediate-early gene of HSV-1 was analyzed with RNAi technology. Two sequences of siRNAs (siRNA-1 and siRNA-2) were prepared by in vitro transcription, and transfected into the Hep-2 and KMB17 cells. The cells were subsequently inoculated with HSV-1. The results demonstrated that there was no differences between the transfected Hep-2 and the untransfected cells. Meanwhile, KMB-17, a kind of restricted cell, which was transfected respectively or co-transfected with siRNA-1 and siRNA-2, showed delayed cytopathic effects with the peak titre of offspring virus appearing later. The results suggested the two siRNAs can interfere with the expression of ICP22 and further effect on the replication of HSV-1.

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    Analysis of siRNA Interfering in theα22 Immediate-early Gene of HSV-1

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    Abstract: In this study, α22, an immediate-early gene of HSV-1 was analyzed with RNAi technology. Two sequences of siRNAs (siRNA-1 and siRNA-2) were prepared by in vitro transcription, and transfected into the Hep-2 and KMB17 cells. The cells were subsequently inoculated with HSV-1. The results demonstrated that there was no differences between the transfected Hep-2 and the untransfected cells. Meanwhile, KMB-17, a kind of restricted cell, which was transfected respectively or co-transfected with siRNA-1 and siRNA-2, showed delayed cytopathic effects with the peak titre of offspring virus appearing later. The results suggested the two siRNAs can interfere with the expression of ICP22 and further effect on the replication of HSV-1.

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