Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain

The DNA component 5 (DNA-5) of Banana bunchy top virus NS strain was cloned and sequenced. It was 1014nts in full length and could code for 146 amino acids. The nucleotide and amino acid sequences of the DNA-5 were compared with those of isolates from Australia, Hawaii, Egypt, and identities of nucleotide and amino acid sequence among these isolates were between 88%~89% and 80%~88%respectively. The secondary structure near the “LXCDE” motif was much different between NS strain and others. It was deduced that efficiency of binding Rb protein would be influenced by this kind of difference.