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Volume 20 Issue 3
June 2005
    Citation: WANG Rui, WU Yun-feng*, HAO Xing-an, FU Hong-qi, YANG Dong, FAN Bing. Sequence Analysis of the HC-pro gene of Potato Virus Y N strain and its Expression in E .coli .VIROLOGICA SINICA, 2005, 20(3) : 320-322.
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    Sequence Analysis of the HC-pro gene of Potato Virus Y N strain and its Expression in E .coli

    • 1.

      College of Plant Protect ion Nort hwest A &F University , Shaanxi Key L aboratory for Agricultural Molecular Biotechnology ,Shaanx i , Yangling 712100 , China

    • Available online: 20 June 2005
    • The HC-pro gene was amplified by RT-PCR from total RNA of tobacco leaves infected with a N strain of Potato virus Y in Shaanxi, and cloned into the PMD 18-T vector. This HC-pro gene is consisted of 1371 nucleotides, encoding 457 amino acids. It shared the sequence homology of 82.5%~96.4 % nucleotide acid and 92.5%~98.0% in amino acids compared to 9 species of PVY N HC-pro abroad.The HC-pro gene was inserted into prokaryotic expressing vector pBV221, to obtain pBVHC recombinant plasmid in E.coli BL21. SDS-PAGE indicated that HC-pro proteins are successfully expressed in E.coli, Western blotting analysis demonstrated that the antibody against the expressed HC-pro can be used to identify the infected plants .
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      Sequence Analysis of the HC-pro gene of Potato Virus Y N strain and its Expression in E .coli

      • 1. College of Plant Protect ion Nort hwest A &F University , Shaanxi Key L aboratory for Agricultural Molecular Biotechnology ,Shaanx i , Yangling 712100 , China

      Abstract: The HC-pro gene was amplified by RT-PCR from total RNA of tobacco leaves infected with a N strain of Potato virus Y in Shaanxi, and cloned into the PMD 18-T vector. This HC-pro gene is consisted of 1371 nucleotides, encoding 457 amino acids. It shared the sequence homology of 82.5%~96.4 % nucleotide acid and 92.5%~98.0% in amino acids compared to 9 species of PVY N HC-pro abroad.The HC-pro gene was inserted into prokaryotic expressing vector pBV221, to obtain pBVHC recombinant plasmid in E.coli BL21. SDS-PAGE indicated that HC-pro proteins are successfully expressed in E.coli, Western blotting analysis demonstrated that the antibody against the expressed HC-pro can be used to identify the infected plants .

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