Studies on the Inhibitory Effects of HNP1,3 on HSV1 Infection in vitro

In order to evaluate the effect of human neutrophil peptide_{1,3}(HNP_{1,3})and acyclovir (ACV) on Herpes simplex virus type 1 (HSV-1) infection, Vero cells were inoculated with HSV-1. Antiviral activity was evaluated by reduction in cytopathic effect and HSV-1 envelop glycoprotein antigen,which determined by sandwich ELISA 48h after infection when drug~3 were interacted with free virus particles and infected vero cells(group A: inactivation of viruses, group B: inhibition of viral replication). Their cytoxicity were also measured by MTT assay. The results showed HNP_{1,3} reversed HSV-1 induced cytopathic effect and inhibited the antigen expression in group A, their 50% efficiency concentrations ( EC_{50}) were 8.1μg/mL、10.03μg/mL;In group B,ACV can lessen HSV-1 induced cytopathicity effects and reduced the antigen secretion whose EC_{50} was 0.68μg/mL. No cytotoxic effects of HNP_\{1,3\} were found by MTT assay. These findings confirmed that HNP_{1,3}, previously showed active to bacterium and human immunod