Citation: XIE Li, HUANG De-zhuang, HE Li-xiang, ZHOU Yu-sen, . Expression,Purification and Antigenicity Analysis of the HDV Antigen .VIROLOGICA SINICA, 2005, 20(4) : 444-446.

Expression,Purification and Antigenicity Analysis of the HDV Antigen

  • Available online: 20 August 2005
  • Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.

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    Expression,Purification and Antigenicity Analysis of the HDV Antigen

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    Abstract: Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.

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