Cloning of Porcine Endogenous Retrovirus 5￠-Untranslated Region and Analysis of Its Structure

The full-length 5′-untranslated region(5′UTR) of Porcine endogenous retrovirus(PERV) from Chinese Wuzhi shan(WZS) miniature pigs was cloned by RACE.Seguencing of PERV-WZS 5′UTR showed that it was 1035bp.Compared with other PERV 5’UTR sequences published in GenBank,the homology was 82.6~94.8%.Primary structure analysis revealed that the PERV-WZS 5′UTR was composed of U3,R,U5,primer binding site(PBS) and leader sequence.The potential promoter and enhancer of the 39-bp repeat sequences were located in U3 region(-67~+1) and(-97~-59) respectively.There were 31 potential cis-acting elements such as NF-Y,TBP,Oct-1,HSF,GATA-1,and GATA-2,which were considered to be related to PERV transcription and regulation.