Transformation of vp1 Gene of Foot-and-Mouth Disease Virus in Legume Forage Lotus cornicu- latus L

The cotyledons and cotyledon petioles of legume forage Lotus corniculatus L.were transformed by Agrobacterium tumefaciens-mediated method with the binary expression vector pBinFMDV-VP1,which contained the vp1gene of FMDV strain Akesu/58 serotype O.Kanamycin-resistant plants were obtained after selected with 50 mg/L kanamycin.The vp1gene was analyzed through PCR with total DNA and through RT-PCR with total RNA from fresh leaves tissue of the kanamycin-resistant lines,the random samples from positive Lotus corniculatus L.lines were analyzed by Western-blotting.These results showed that the vp1 gene was transformed into Lotus corniculatus L.plants and had transcription activity;the VP1 protein was expressed correctly.The transgenic lines was transplanted to greenhouse,and further work is under way.