Citation: HUANG Juan, JIANG Ping, ZHANG Chang-yin, TANG Tai-shan, LI Yong-dong, ZHANG Zhi-tao. Development of Real-time PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus .VIROLOGICA SINICA, 2005, 20(5) : 530-533.

Development of Real-time PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus

  • Available online: 20 October 2005
  • Primers and probe were designed from the nucleotide sequence within the ORF7 of Porcine reproductive and respiratory syndrome virus(PRRSV),and a quantitative TaqMan real-time PCR was developed for PRRSV detection.Specificity and sensitivity were compared with virus isolation and a conventional RT-PCR,and were found to be equal or superior to the reference methods.Reproducibility was tested and proved that the assay was reliable.Standard dilutions allowed absolute quantitation of the amount of viral RNA.The TaqMan real-time PCR described below is time-saving,easy to handle,exhibits a decreased risk of cross-contamination and is highly sensitive and specific.It is considered to be a powerful tool for the rapid detection of PRRSV.

  • 加载中
  • 加载中

Article Metrics

Article views(4227) PDF downloads(20) Cited by()

Related
Proportional views

    Development of Real-time PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus

    • 1. 

    Abstract: Primers and probe were designed from the nucleotide sequence within the ORF7 of Porcine reproductive and respiratory syndrome virus(PRRSV),and a quantitative TaqMan real-time PCR was developed for PRRSV detection.Specificity and sensitivity were compared with virus isolation and a conventional RT-PCR,and were found to be equal or superior to the reference methods.Reproducibility was tested and proved that the assay was reliable.Standard dilutions allowed absolute quantitation of the amount of viral RNA.The TaqMan real-time PCR described below is time-saving,easy to handle,exhibits a decreased risk of cross-contamination and is highly sensitive and specific.It is considered to be a powerful tool for the rapid detection of PRRSV.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return