Molecular characterization of the genome segment S9 of Rice gall dwarf virus
Abstract: The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same genomic organization as that of RGDV Thailand isolate(RGDV-T) and shared 98.1% nucleotide and 98.5% amino acid sequence identity.No equivalent protein of Pns9 encoded by RGDV S9 ORF was found in other members of the genus Phythoreovirus.Pns9 shared 21.8% amino acid sequence identity with ATP-dependent Clp protease proteolytic component from Borrelia burgdorferi.