Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan

Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses(WTGV),PCR product of expected size was amplified from six samples.The specific fragments were cloned and sequenced,and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST.Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity.Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG,P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch.