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Volume 20 Issue 6
December 2005
    Citation: TIAN Yong-jun, QIN Li, ZHANG Zheng-mao, LI Lei, LEI Yan-chang, YU Zhi-qun, ZHAO Xi-ping, YANG Dong-liang*. Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg .VIROLOGICA SINICA, 2005, 20(6) : 574-577.
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    Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg

    • 1.

    • Available online: 20 December 2005
    • A recombinant plasmid named pCI-HBs145 containing a fragment of G145R mutate S gene of Hepatitis B virus was constructed. Seven HeLa cell lines stablely expressing the protein of G145R HBsAg were developed by G418 selection after transfecting with the PCI-HBs145. Biological characteristics of one of the 7 cell lines, namely, 2A8 were studied. The immunoreactivity of G145R HBsAg expressed by 2A8 was different from wild type HBsAg. The genetic stability and purity of the cell population were confirmed. The kinetics and optimal conditions for mutation HBsAg secretion from 2A8 cells were studied.
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      Study on a Transgenic Cell Line Stable Expression Gly145Arg Mutation HBsAg

      • 1. 

      Abstract: A recombinant plasmid named pCI-HBs145 containing a fragment of G145R mutate S gene of Hepatitis B virus was constructed. Seven HeLa cell lines stablely expressing the protein of G145R HBsAg were developed by G418 selection after transfecting with the PCI-HBs145. Biological characteristics of one of the 7 cell lines, namely, 2A8 were studied. The immunoreactivity of G145R HBsAg expressed by 2A8 was different from wild type HBsAg. The genetic stability and purity of the cell population were confirmed. The kinetics and optimal conditions for mutation HBsAg secretion from 2A8 cells were studied.

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