Citation: ZHANG Xiao-xia, LIANG Zhen-pu, PENG Hui-yin, ZHANG Zhong-xin. Construction and Immunogenicity of Recombinant Canine Adenovirus Type 2 of Feline Distemper Virus VP2 .VIROLOGICA SINICA, 2005, 20(6) : 660-663.

Construction and Immunogenicity of Recombinant Canine Adenovirus Type 2 of Feline Distemper Virus VP2

  • Available online: 20 December 2005
  • Primers based on published sequences of granulin genes were synthesized and used to amplify the granulin gene from Clostera anachoreta granulovirus (ClanGV). The generated product was se-quenced. Sequence analysis indicated the entire ORF of ClanGV granulin gene was 747bp, encoding a protein of 248 aa with an estimated molecular weight of 29. 3kDa. A bacluloviral late promoter was present at 24 bp upstream of the ATG condon. Two TATA boxes were at 26 and 65bp upstream of the ATG codon, respectively. Phylogenetic analysis indicated that ClanGV was closely related to Adoxophyes ora-na granulovirus (AoGV)、Cydia pomonella granulovirus (CpGV)、Choristoneura fumiferana granulovirus (CfGV)、and Phthorimaea operculella granulovirus (PoGV). Antibodies prepared against the protein were used in Western blot analysis and indicated that ClanGV granulin reacted positively with occlusion body proten from HaSNPV and CaLGV and reacted weakly with polyhedrin of SeMNPV.

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    Construction and Immunogenicity of Recombinant Canine Adenovirus Type 2 of Feline Distemper Virus VP2

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    Abstract: Primers based on published sequences of granulin genes were synthesized and used to amplify the granulin gene from Clostera anachoreta granulovirus (ClanGV). The generated product was se-quenced. Sequence analysis indicated the entire ORF of ClanGV granulin gene was 747bp, encoding a protein of 248 aa with an estimated molecular weight of 29. 3kDa. A bacluloviral late promoter was present at 24 bp upstream of the ATG condon. Two TATA boxes were at 26 and 65bp upstream of the ATG codon, respectively. Phylogenetic analysis indicated that ClanGV was closely related to Adoxophyes ora-na granulovirus (AoGV)、Cydia pomonella granulovirus (CpGV)、Choristoneura fumiferana granulovirus (CfGV)、and Phthorimaea operculella granulovirus (PoGV). Antibodies prepared against the protein were used in Western blot analysis and indicated that ClanGV granulin reacted positively with occlusion body proten from HaSNPV and CaLGV and reacted weakly with polyhedrin of SeMNPV.

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