Citation: DAI Wen-tao, DENG Fei, WANG Hua-lin, YUAN Li, HU Zhi-hong. The Transcription Profiles of Nine HaSNPV Genes .VIROLOGICA SINICA, 2006, 21(1) : 47-51.

The Transcription Profiles of Nine HaSNPV Genes

  • Corresponding author: HU Zhi-hong, 
  • Available online: 20 January 2006
  • The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes the transcriptional start point was coincident with the prediction of their promoter. But the predicted early phase gene pkip started to be transcribed at late phase, and the predicted early and late phase gene ha107 was only transcribed at late phase. At 72hpi, the transcription level of these genes was generally the highest. The transcription level of polyhedrin was obviously higher than the other genes. The iap2 was the second highly transcribed gene, indicating that it might be functional. The HaSNPV p10, unlike its homologue in AcMNPV, was not highly transcribed.

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    The Transcription Profiles of Nine HaSNPV Genes

      Corresponding author: HU Zhi-hong,
    • 1. 1. State Key Laboratory of Virology, Key Laboratory of Molecular Virology, and Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    • 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China

    Abstract: The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes the transcriptional start point was coincident with the prediction of their promoter. But the predicted early phase gene pkip started to be transcribed at late phase, and the predicted early and late phase gene ha107 was only transcribed at late phase. At 72hpi, the transcription level of these genes was generally the highest. The transcription level of polyhedrin was obviously higher than the other genes. The iap2 was the second highly transcribed gene, indicating that it might be functional. The HaSNPV p10, unlike its homologue in AcMNPV, was not highly transcribed.

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