Citation: NI Fang, LU Zhuo-zhuang, WANG Li-sheng, QU Cheng-kui, WANG Si-ying. Construction of a Novel Retargeted Adenoviral Vector with High Gene Transfer Efficiency into Hematopoietic Cells .VIROLOGICA SINICA, 2006, 21(2) : 106-110.

Construction of a Novel Retargeted Adenoviral Vector with High Gene Transfer Efficiency into Hematopoietic Cells

  • Corresponding author: WANG Si-ying, 
  • Available online: 20 March 2006
  • The adenovirus subgenus C serotypes 5 (Ad5) vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. An Ad5-based vector encoding the green fluorescent protein (GFP) gene under the control of the CMV promoter with Ad11p fiber receptor specificity was generated (Ad5F11p-GFP). The Ad5F11p-GFP vector-mediated gene transfer efficiency for some committed hematopoietic cell lines such as myeloblasts (K562) and monocytes (U937) was evaluated using flow cytometry and compared to that of Ad5-GFP, which also encodes the GFP gene under the control of the CMV promoter. The results showed that these cell lines were superiorly transduced by the Ad5F11p-GFP vector at the same MOI (multiplicity of infection, MOI) compared with the Ad5-GFP vector,more than 90% tested cells were infected by Ad5F11p-GFP adenovirus while less than 30% cells were infected by Ad5-GFP at 10 MOI.

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    Construction of a Novel Retargeted Adenoviral Vector with High Gene Transfer Efficiency into Hematopoietic Cells

      Corresponding author: WANG Si-ying,
    • 1. Department of Pathophysiology, Anhui Medical University, Hefei 230032, China
    • 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China

    Abstract: The adenovirus subgenus C serotypes 5 (Ad5) vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. An Ad5-based vector encoding the green fluorescent protein (GFP) gene under the control of the CMV promoter with Ad11p fiber receptor specificity was generated (Ad5F11p-GFP). The Ad5F11p-GFP vector-mediated gene transfer efficiency for some committed hematopoietic cell lines such as myeloblasts (K562) and monocytes (U937) was evaluated using flow cytometry and compared to that of Ad5-GFP, which also encodes the GFP gene under the control of the CMV promoter. The results showed that these cell lines were superiorly transduced by the Ad5F11p-GFP vector at the same MOI (multiplicity of infection, MOI) compared with the Ad5-GFP vector,more than 90% tested cells were infected by Ad5F11p-GFP adenovirus while less than 30% cells were infected by Ad5-GFP at 10 MOI.

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