Expression of Porcine Parvovirus vp2 Gene and Construction of Virus Like Particles
Abstract: In this study, the open reading frame of PPV vp2 was amplified by PCR, sequenced, cloned and expressed both in prokaryotic and insect cells. In E. coli, by using pET-32(a) expression vector, VP2 was expressed as a Trx-His-S Tag fusion protein with the size of 84 kDa and the protein was used to immunize rabbits for generating specific antibody. Moreover, vp2 gene was cloned into pFastBacDUAL and expressed in baculovirus/insect cell expression system: AcMNPV Bac-to-Bac system. SDS-PAGE and Western-blot analysis showed a single band with the size of 64 kDa. In Sf-21 cells, virus like particles could be observed under electron microscope.