Citation: CHEN Hong-jun, SONG Cui-ping, QIN Ai-jian*, LIU Yue-long, JIN Wen-jie. Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli .VIROLOGICA SINICA, 2006, 21(2) : 169-172.

Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli

  • Available online: 20 March 2006
  • Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22.

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    Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli

    • 1. Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

    Abstract: Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22.

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